Abstract : The insect gut is home to an extensive array of microbes that play a crucial role in the
digestion and absorption of nutrients, as well as in the protection against pathogenic microorganisms.
The variety of these gut microbes is impacted by factors such as age, diet, pesticides, antibiotics, sex,
and caste. Increasing evidence indicates that disturbances in the gut microbiota can lead to compro-
mised insect health, and that its diversity has a far-reaching impact on the host’s health. In recent
years, the use of molecular biology techniques to conduct rapid, qualitative, and quantitative research
on the host intestinal microbial diversity has become a major focus, thanks to the advancement of
metagenomics and bioinformatics technologies. This paper reviews the main functions, influencing
factors, and detection methods of insect gut microbes, in order to provide a reference and theoretical
basis for better research utilization of gut microbes and management of harmful insects.