La recherche italienne à la manœuvre....
vendredi 20 septembre 2019 -
Nos amis italiens sont réellement concernés par le charançon rouge ( aucune ironie dans ce propos !) puisqu’ils y consacrent cet article, scientifique, publié ce jour à la signature de professeurs de l’université Aldo Moro à Bari.
Si on sait que la principale implantation du CIHEAM est justement à Bari on comprend mieux que nous ne sommes pas les mieux placés pour faire prévaloir les atouts de la recherche française qui est en dehors du Paysandisia ne consacre plus au CRP le moindre € !
The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce. Here, we report a comprehensive characterisation of CRISPR-Cas systems identified in S. marcescens strains isolated as secondary symbionts of Rhynchophorus ferrugineus, also known as Red Palm Weevil (RPW), one of the most invasive pests of major cultivated palms. Whole genome sequencing was performed on four strains (S1, S5, S8, and S13), which were isolated from the reproductive apparatus of RPWs. Subtypes I-F and I-E were harboured by S5 and S8, respectively. No CRISPR-Cas system was detected in S1 or S13. Two CRISPR arrays (4 and 51 spacers) were detected in S5 and three arrays (11, 31, and 30 spacers) were detected in S8. The CRISPR-Cas systems were located in the genomic region spanning from ybhR to phnP, as if this were the only region where CRISPR-Cas loci were acquired. This was confirmed by analyzing the S. marcescens complete genomes available in the NCBI database. This region defines a genomic hotspot for horizontally acquired genes and/or CRISPR-Cas systems. This study also supplies the first identification of subtype I-E in S. marcescens.